Rat GDF-15 ELISA Kit

$515.00

The Rat GDF-15 ELISA Kit is intended for the quantitation of Rat GDF15 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Rat GDF-15 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: RGF21-K01 Categories: , ,

Rat GDF-15 ELISA Kit

The Rat GDF-15 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 15.6pg/ml-1000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Names: Growth Differentiation Factor 15, TGF-PL, MIC-1, PLAB, and PTGFB


Assay Principle

The Rat Gdf15 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Gdf15 with a 96-well strip plate that is pre-coated with antibody specific for Gdf15. The detection antibody is a biotinylated antibody specific for Gdf15. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Gdf15 with immunogen: Expression system for standard: NSO; Immunogen sequence: S189-A303. The kit is analytically validated with ready to use reagents. To measure Rat Gdf15, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Gdf15 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Rat Gdf15 in the sample.


Related Products

Mouse GDF-15 ELISA Kit
Human GDF-15 ELISA Kit
Rat NCAM1/CD56 ELISA Kit

Additional Information

Assay Background


GDF-15(Growth differentiation factor 15), also known as TGF-PL, MIC-1, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

Typical Standard Curve


Documents

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