Rat Flt-3 ligand ELISA Kit

$495.00

The Rat FLT-3ligand ELISA Kit is intended for the quantitation of Rat FLT3LG concentrations in cell culture supernates and serum. The Eagle Biosciences Rat FLT-3ligand ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: FT321-K01 Categories: , ,

Rat Flt-3 ligand ELISA Kit

The Rat Flt-3 ligand ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates and Serum
Sample Size: 100 µl
Alternative Names: Fms-related tyrosine kinase 3 ligand, FLT3LG


Assay Principle

The Rat Flt3lg Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Flt3lg with a 96-well strip plate that is pre-coated with antibody specific for Flt3lg. The detection antibody is a biotinylated antibody specific for Flt3lg. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Flt3lg with immunogen: Expression system for standard: NSO, Immunogen sequence: G27-R188. The kit is analytically validated with ready to use reagents. To measure Rat Flt3lg, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Flt3lg in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Rat Flt3lg in the sample.


Related Products

Rat FLT4 ELISA Kit
Human Flt-3ligand ELISA Kit
Mouse Flt-3ligand ELISA Kit

Additional Information

Assay Background


Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells.

Typical Standard Curve


Rat Flt-3 ligand ELISA Kit

Documents

Product Documents