Progesterone LIA Assay Kit

$190.00

The Progesterone LIA Assay Kit is for the direct quantitative determination of progesterone in human serum by a chemiluminescence immunoassay (LIA). The Eagle Biosciences Progesterone LIA assay kit is for research use only and not to be used in diagnostic procedures.

SKU: PRG31-L01 Categories: , ,

Progesterone LIA Assay Kit

The Progesterone LIA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 ng/mL
Dynamic Range: 0.1–60 ng/mL
Incubation Time: 90 minutes
Sample Type: Serum
Sample Size: 25 μL
Controls Included


Assay Principle

The principle of the Progesterone LIA Assay Kit chemiluminescence immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of progesterone in the sample. A set of calibrators are used to plot a standard curve from which the amount of progesterone in patient samples and controls can be directly read.


SPECIMEN COLLECTION AND STORAGE
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
POTENTIAL BIOHAZARDOUS MATERIAL
Human serum that may be used in the preparation of the standards and controls has been tested and found to be nonreactive for Hepatitis B surface antigen and has also been tested for the presence of antibodies to HCV and Human Immunodeficiency Virus (HIV) and found to be negative. No test method however, can offer complete assurance that HIV, HCV and Hepatitis B virus or any infectious agents are absent. The reagents should be considered a potential biohazard and handled with the same precautions as applied to any blood specimen.


Related Products

Progesterone ELISA Assay Kit
17OH Progesterone ELISA Assay Kit
Cortisol LIA Assay Kit

Additional Information

Assay Background


Progesterone is a C-21 female sex steroid hormone with a variety of physiological effects. In the follicular phase of the menstrual cycle, progesterone is produced in low levels. It increases to the LH peak and then sharply rises 3 to 4 days later to higher levels, remaining elevated through the 10th to 12th days after the LH peak. Then there is a sharp decline to the low levels of the follicular phase. It is responsible for the induction of the cyclic changes in the endometrium of the uterus allowing implantation and successful growth of the fertilized ovum and maintenance of pregnancy. Progesterone measurements are used in documenting ovulation and in the management of difficulties during the first trimester of pregnancy. Levels of progesterone may be useful in the evaluation of sterility due to luteal phase defects, prediction of impending abortion, and the diagnosis of ectopic pregnancy. Drugs such as oral contraceptives, superovulatory drugs, estrogen replacement therapy medication, and GnRH analogues may affect normal values of progesterone. The removal of ovarian function following surgical oophorectomy or chemotherapy may influence serum progesterone values.

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