Cortisol LIA Assay Kit
The Cortisol LIA Assay Kit is manufactured in the United States of America
Size: 1×96 wells
Sensitivity: 0.15 μg/dL
Dynamic Range: 0.5–60 μg/dL
Incubation Time: 90 minutes
Sample Type: Serum
Sample Size: 50 μL
For Research Use Only
Controls Included
SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
Specificity (Cross Reactivity)
The following compounds were tested for cross-reactivity with the cortisol LIA kit with cortisol
cross-reacting at 100%:
Prednisolone 13.6%
Corticosterone 7.6%
Deoxycorticosterone 7.2%
Progesterone 7.2%
Cortisone 6.2%
Deoxycortisol 5.6%
Pednisone 5.6%
Dexamethasone 1.6%
No cross-reaction was detected with DHEAS and Tetrahydrocortisone.
Please note that there is an observed cross-reactivity of 13.6% with prednisolone. Since prednisone is converted to prednisolone in vivo, caution must be exercised when assaying the cortisol levels of patients undergoing either therapy.
Assay Principle
The principle of the following chemiluminescence immunoassay (LIA) test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of cortisol in the sample. A set of calibrators are used to plot a standard curve from which the amount of cortisol in patient samples and controls can be directly read.
Related Products
DHEAS LIA Assay Kit
Progesterone LIA Assay Kit
Estrone LIA Assay Kit
