Cortisol LIA Assay Kit
The Cortisol LIA Assay Kit is manufactured in the United States of America
Size: 1×96 wells
Sensitivity: 0.15 μg/dL
Dynamic Range: 0.5–60 μg/dL
Incubation Time: 90 minutes
Sample Type: Serum
Sample Size: 50 μL
For Research Use Only
SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
Specificity (Cross Reactivity)
The following compounds were tested for cross-reactivity with the cortisol LIA kit with cortisol
cross-reacting at 100%:
No cross-reaction was detected with DHEAS and Tetrahydrocortisone.
Please note that there is an observed cross-reactivity of 13.6% with prednisolone. Since prednisone is converted to prednisolone in vivo, caution must be exercised when assaying the cortisol levels of patients undergoing either therapy.
Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. Measurement of blood cortisol levels can be used as an indicator of adrenal function and the differential diagnosis of Addison’s and Cushing’s diseases as well as adrenal hyperplasia and carcinoma. Most circulating cortisol is bound to cortisol binding globulin or transcortin. Therefore, the free cortisol concentration excreted in the urine is very small, and the 24-hour collection of urine is a must in order to obtain an accurate measurement of urinary cortisol. Cortisol in blood shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.