Cortisol LIA Assay Kit


The Cortisol LIA Assay Kit is for the direct quantitative determination of cortisol in human serum by a chemiluminescence immunoassay (LIA). The Eagle Biosciences cortisol LIA assay kit is for research use only and not to be used in diagnostic procedures.

SKU: COR31-L01 Categories: , ,

Cortisol LIA Assay Kit

The Cortisol LIA Assay Kit is manufactured in the United States of America

Size: 1×96 wells
Sensitivity: 0.15 μg/dL
Dynamic Range: 0.5–60 μg/dL
Incubation Time: 90 minutes
Sample Type: Serum
Sample Size: 50 μL
For Research Use Only

Controls Included

Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
Specificity (Cross Reactivity)
The following compounds were tested for cross-reactivity with the cortisol LIA kit with cortisol
cross-reacting at 100%:
Prednisolone 13.6%
Corticosterone 7.6%
Deoxycorticosterone 7.2%
Progesterone 7.2%
Cortisone 6.2%
Deoxycortisol 5.6%
Pednisone 5.6%
Dexamethasone 1.6%
No cross-reaction was detected with DHEAS and Tetrahydrocortisone.
Please note that there is an observed cross-reactivity of 13.6% with prednisolone. Since prednisone is converted to prednisolone in vivo, caution must be exercised when assaying the cortisol levels of patients undergoing either therapy.

Assay Principle

The principle of the following chemiluminescence immunoassay (LIA) test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of cortisol in the sample. A set of calibrators are used to plot a standard curve from which the amount of cortisol in patient samples and controls can be directly read.

Related Products

Progesterone LIA Assay Kit
Estrone LIA Assay Kit

Additional Information

Assay Background

Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. Measurement of blood cortisol levels can be used as an indicator of adrenal function and the differential diagnosis of Addison’s and Cushing’s diseases as well as adrenal hyperplasia and carcinoma. Most circulating cortisol is bound to cortisol binding globulin or transcortin. Therefore, the free cortisol concentration excreted in the urine is very small, and the 24-hour collection of urine is a must in order to obtain an accurate measurement of urinary cortisol. Cortisol in blood shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations