Total PSA LIA Assay
The Total PSA LIA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.082 ng/mL
Dynamic Range: 0.1–100 ng/mL
Incubation Time: 120 minutes
Sample Type: Serum
Sample Size: 50 μL
Alternative Names: Total Prostate Specific Antigen LIA, Total Human PSA LIA, Total Prostatic Specific Antigen LIA
The principle of this chemiluminescence immunoassay (LIA) test follows a typical one-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for total PSA is immobilized onto the microplate and another monoclonal antibody specific for a different region of PSA is conjugated to horse radish peroxidase (HRP). Total PSA from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the luminescence substrate is added and the relative luminescence units (RLUs) are measured in a microplate luminometer. The RLUs formed by the enzymatic reaction are directly proportional to the concentration of total PSA in the samples. A set of standards is used to plot a standard curve from which the concentrations of tPSA in patient and control samples are read.
SPECIMEN COLLECTION AND STORAGE
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
This assay is a direct system; no specimen pretreatment is necessary.