17OH Progesterone ELISA Assay Kit

$190.00

The 17OH Progesterone ELISA Kit is intended for the direct quantitative determination of 17α-hydroxyprogesterone (17α-OHP) in human serum. The 17OH Progesterone ELISA Assay kit is for research use only and not to be used in diagnostic procedures.

SKU: P1731-K01 Categories: , ,

17OH Progesterone ELISA Assay Kit

The 17OH Progesterone ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.11 ng/mL
Dynamic Range: 0.15–20 ng/mL
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 50 μL
Controls Included


Assay Principle

The principle of the 17OH Progesterone ELISA Assay Kit enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed is inversely proportional to the concentration of 17α-OHP in the sample. A set of standards is used to plot a standard curve from which the amount of 17α-OHP in patient samples and controls can be directly read.


SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
POTENTIAL BIOHAZARDOUS MATERIAL
Human serum that may be used in the preparation of the standards and controls has been tested and found to be nonreactive for Hepatitis B surface antigen and has also been tested for the presence of antibodies to HCV and Human Immunodeficiency Virus (HIV) and found to be negative. No test method however, can offer complete assurance that HIV, HCV and Hepatitis B virus or any infectious agents are absent. The reagents should be considered a potential biohazard and handled with the same precautions as applied to any blood specimen.


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Additional Information

Assay Background


The steroid 17α-hydroxyprogesterone is produced by the adrenal cortex and gonads. 17α-OHP has little progestational activity, but it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Cpd-S is produced by the 21-hydroxylation of 17α-OHP. Measurement of 17α-OHP is a useful indirect indicator of 21-hydroxlase activity. In congenital 21-hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17α-OHP is secreted in abundant excess. Measurement of 17α-OHP is therefore valuable in the initial diagnosis of CAH.

Typical Standard Curve


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