17OH Progesterone ELISA Assay Kit

$230.00

The Eagle Biosciences 17OH Progesterone ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of 17OH Progesterone concentration in human serum or plasma. The 17OH Progesterone ELISA Assay Kit is intended for research use only and not to be used in diagnostic procedures.

SKU: DCM004 Categories: , ,

17OH Progesterone ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.09 ng/mL
Dynamic Range: 0.2 – 19.2 ng/ml
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 25 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

17-Hydroxyprogesterone (17OH Progesterone or 17α-OHP or 17-OHP) is a C-21 steroid hormone produced in the adrenal gland and gonads, during the synthesis of glucocorticoids and sex steroids. It is derived from progesterone via 17-hydroxylase, a P450c17 enzyme, or from 17-hydroxypregnenolone via 3β-hydroxysteroid dehydrogenase/Δ5-4 isomerase.  17-OHP has no defined physiologic role except as a precursor molecule.

Serum 17-OHP levels are age-dependent, with peak levels observed during fetal life and the immediate postnatal period. During the first week of life, serum 17 -OHP levels fall ~50-fold as compared to cord blood values. A small transient increase occurs in male infants 30-60 days postnatally. Levels for both sexes remain at constant low levels during childhood, and then progressively increase during puberty reaching adult levels of ~100 ng/dL (~3.03 nmol/L). As with cortisol, serum 17-OHP levels normally have an ACTH-dependent diurnal variation, with peak levels in the morning and a nadir at night. In addition, ovarian production of 17-OHP increases during the luteal phase of the menstrual cycle.

17-hydroxyprogesterone is a natural progestin and in pregnancy increases in the third trimester primarily due to fetal adrenal production.  Normal levels are 3-90 ng/dl in children, and in women, 15-70 ng/dl prior to ovulation, and 35-290 ng/dl during the luteal phase. Measurements of levels of 17-hydroxyprogesterone are useful in the evaluation of patients with suspected congenital adrenal hyperplasia as the typical enzymes that are defective, namely 21-hydroxylase and 11b-hydroxilase, lead to a build-up of 17-OHP. In contrast, the rare patient with 17α-hydroxylase deficiency will have very low or undetectable levels of 17-OHP.  Elevated serum 17-OHP levels at baseline and/or after ACTH stimulation have also been reported in other forms of adrenal hyperplasia.

Assay Principle

17OH Progesterone (antigen) in the sample competes with the antigenic 17OH Progesterone conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti 17OH Progesterone coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blu color that changes into yellow when the Stop Solution (H2SO4) is added. The colour intensity is inversely proportional to the 17OH Progesterone concentration  in the sample. 17OH Progesterone concentration in the sample is calculated through a calibration curve.

Manual

Product Manual


Publications

Citations

Irani, M;Seifer, DB;Grazi, RV;Irani, S;Rosenwaks, Z;Tal, R;, (2017). Vitamin D Decreases Serum VEGF Correlating with Clinical Improvement in Vitamin D-Deficient Women with PCOS: A Randomized Placebo-Controlled Trial. Nutrients, 9(4), PubMed:28350328.