Thrombospondin 2 ELISA Kit


The Thrombospondin 2 ELISA Kit is designed for quantification of human TSP-2 in serum and plasma samples. The Eagle Biosciences Thrombospondin 2 ELISA Kit is for research use only and not to be used in diagnostic procedures.

SKU: TSP31-K01 Categories: , ,

Thrombospondin 2 ELISA Kit

Thrombospondin 2 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.156ng/ml
Standard Range: 0.156ng/ml-10ng/ml
Incubation Time: 3 hours 35 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl
Alternative Names: TSP-2

Assay Principle

The Thrombospondin 2 (TSP-2) assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TSP-2. Standards and samples are pipetted into the wells and any human TSP-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labeled polyclonal antibody specific for human TSP-2 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TSP-2, the unknown sample concentration can be interpolated from a reference curve included in each assay kit.

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Additional Information

Assay Background

Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein. TSP-2 inhibits angiogenesis through direct effect on endothelial cell migration, proliferation, survival and apoptosis. It is a potential risk factor in heart disease.


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