DKK-1 ELISA Assay Kit
DKK-1 ELISA Assay Kit Developed and Manufactured in Austria by Biomedica
Size: 1×96 wells
Sensitivity: LOD: 1.7 pmol/l (0 pmol/l + 3 SD) , LLOQ: 1.25 pmol/l
Dynamic Range: 0 to 160 pmol/l
Incubation Time: 3.5 hours
Sample Type: Human serum
Sample Size: 20 µL
Alternative Names: DKK-1 ELISA, DKK1 ELISA, Dickkopf-1 ELISA, Human Dickkopf-1 ELISA
For Research Use Only
Unit conversion: 1 pg/ml = 0.039 pmol/l (MW = 25.8 kDa)
Reference Range: Median Serum (n=51)= 34 pmol/l
Each laboratory should establish its own reference range for the samples under investigation.
Specificity: oligomeric forms of natural and recombinant human DKK-1
The DKK-1 ELISA Assay kit is a sandwich enzyme immunoassay for the quantitative determination of DKK-1 in human serum samples. In a first step, assay buffer, standard/control/sample and detection antibody (biotinylated polyclonal rabbit anti-human DKK-1) are pipetted into the wells of the microtiter strips, which are pre-coated with monoclonal mouse anti-human DKK-1 antibody. DKK-1 present in the standard/control/sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. In the washing step all non-specific unbound material is removed. In a next step, the conjugate (streptavidin-HRP) is pipetted into the wells and reacts with the detection antibody. After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of DKK-1 present in the sample. This color change is detectable with a standard microtiter plate reader. A dose response curve of the absorbance (optical density, OD at 450 nm) versus standard concentration is generated using the values obtained from the standard. The concentration of DKK-1 in the sample is determined directly from the dose response curve.