Human Periostin ELISA Assay Kit
Periostin ELISA Assay Kit Developed and Manufactured in Austria by Biomedica
Size: 1×96 wells
Sensitivity: LOD: 20 pmol/l (0 pmol/l + 3 SD); LLOQ: 62.5 pmol/l
Dynamic Range: 0 – 4000 pmol/l
Incubation Time: 5.5 hours
Sample Type: Serum, plasma (EDTA, heparin, citrate)
Sample Size: 150 µL
Alternative Names: OSF-2, POSTN, OSF2, PDLPN, PN
For Research Use Only
Unit conversion: 1 pg/ml = 0.011 pmol/l (MW: 91 kDa)
Cross Reactivity: Due to the high sequence homology between human Periostin and Periostin of other species, the antibodies utilized in the assay may cross react with mouse, rat, cynomolgous monkey, dog and cat Periostin.
The Periostin ELISA kit is a sandwich enzyme immunoassay for the quantitative determination of periostin in human serum and plasma samples. In a first step, pre-diluted standard/control/sample and biotinylated polyclonal goat anti-human Periostin antibody are pipetted into the wells of the microtiter strips, which are pre-coated with mouse monoclonal anti-human Periostin antibody. Periostin present in the standard/control/sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. In a washing step all non-specific unbound material is removed. In a second step, the conjugate (streptavidin-HRP) is pipetted into the wells and reacts with the detection antibody. After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of Periostin present in the sample. This color change is detectable with a standard microtiter plate reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standard. The concentration of human Periostin in the sample is determined directly from the dose response curve.