Noradrenaline (Norepinephrine) ELISA Assay Kit

$520.00

The Eagle Biosciences Noradrenaline (Norepinephrine) ELISA Assay Kit is intended for the quantitative determination of Noradrenaline (also known as Norepinephrine) in urine or plasma by a microplate enzyme immunoassay (ELISA). The Noradrenaline (Norepinephrine) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

Noradrenaline (Norepinephrine) ELISA Assay kit

For Research Use Only

Size : 1×96 wells
Sensitivity :  Urine: 0.24 ng/ml, Plasma:  16 pg/ml
Dynamic Range : 1.5 –  500 ng/ml (8.9 – 2955 nmol/L)
Incubation Time : Overnight
Sample Type : Urine, Plasma
Sample Size : 20 µL (Urine), 300 µl (Plasma)

Controls Included

Product Developed and Manufactured in Germany by DLD Diagnostika

Additional Information

Assay Background

Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitters, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumors of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). Studies show that an increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis.

The Noradrenaline (Norepinephrine) ELISA Assay Kit provides materials for the quantitative measurement of noradrenaline in plasma and urine. Noradrenaline is extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline and then converted enzymatically into N-acylnormeta­nephrine.

Assay Principle

The competitive Noradrenaline (Norepinephrine) ELISA Assay Kit uses the microtiter plate format. Noradrenaline is bound to the solid phase of the microtiter plate. Acylated noradrenaline from the sample and solid phase bound noradrenaline compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase noradrenaline is detected by anti-rabbit IgG/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase noradrenaline is inversely proportional to the noradrenaline concentration of the sample.

Typical Standard Curve

Manual

Product Manual


Publications

Citations

Using Human Samples

Campbell, MD “Metabolic implications when employing heavy pre- and post-exercise rapid-acting insulin reductions to prevent hypoglycaemia in type 1 diabetes patients: a randomised clinical trial. “PLoS ONE 2014: 9(5). doi:10.1371/journal.pone.0097143

Kabir AU,et al. “Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters.” PLoS ONE 2015; 10(2): e0116546. doi:10.1371/journal.pone.0116546

Using Rat Samples

Montgomery, LR;Hubscher, CH;, (2017). Altered vasopressin and natriuretic peptide levels in a rat model of spinal cord injury – Implications for the development of polyuria. Am. J. Physiol. Renal Physiol., ajprenal.00229.2017
Sun, H;Li, Q;Liu, Y;Wei, X;Pan, C;Fan, J;Han, J;, (2017). Xiao-Yao-San, a Chinese Medicine Formula, Ameliorates Chronic Unpredictable Mild Stress Induced Polycystic Ovary in Rat. Frontiers in Physiology.

Samad, MB;Mohsin, MNAB;Razu, BA;Hossain, MT;Mahzabeen, S;Unnoor, N;Muna, IA;Akhter, F;Kabir, AU;Hannan, JMA;, (2017). [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Lepr(db/db) type 2 diabetic mice. BMC Complement Altern Med, 17(1), 395

More, AS;Mishra, JS;Hankins, GD;Kumar, S;, (2016). Prenatal Testosterone Exposure Decreases Aldosterone Production but Maintains Normal Plasma Volume and Increases Blood Pressure in Adult Female Rats. Biol. Reprod., 95(2), 42

Nandi, SS;Zheng, H;Sharma, NM;Shahshahan, HR;Patel, KP;Mishra, PK;, (2016). Lack of miR-133a Decreases Contractility of Diabetic Hearts: A Role for Novel Cross Talk Between Tyrosine Aminotransferase and Tyrosine Hydroxylase. Diabetes, 65(10), 3075-3090.

Zhang, C, Role of beta-endorphin in control of stress and cancer progression in fetal alcohol exposed rats, 2013