Adrenaline, Noradrenaline & Dopamine ELISA Assay Kit

$920.00

The Adrenaline, Noradrenaline, & Dopamine ELISA Assay Kit is intended for the quantitative determination of adrenaline, noradrenaline, and dopamine in plasma and urine by microplate immunoassay (ELISA). The Eagle Biosciences Adrenaline, Noradrenaline, & Dopamine ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

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Adrenaline, Noradrenaline & Dopamine Assay kit

Adrenaline, Noradrenaline & Dopamine ELISA Assay Kit Developed and Manufactured in Germany by DLD Diagnostika EA603/288

Size: 3 x 96 wells (1 plate for Adrenaline, 1 plate for Noradrenaline, 1 plate for Dopamine)
Sensitivity:
Urine: Adrenaline: 0.08 ng/ml, Noradrenaline: 0.24 ng/ml, Dopamine 0.44 ng/ml
Plasma: Adrenaline: 5 pg/ml, Noradrenaline: 16 pg/ml, Dopamine 29 pg/ml
Dynamic Range: Adrenaline: 0.5 – 250 ng/ml, Noradrenaline: 1.5 – 500 ng/ml, Dopamine:  1.5 – 2560 ng/ml
Incubation Time:  Overnight
Sample Type: Urine, EDTA Plasma
Sample Size: Urine: 20 µl, Plasma:  300 µl
Alternative Names: Epinephrine, Norepinephrine
For Research Use Only

Controls Included


Normal Values
Urine: Adrenaline < 20 µg/day, Noradrenaline < 90 µg/day, Dopamine < 600 µg/day
Plasma: Adrenaline < 100 pg/ml, Noradrenaline < 600 pg/ml, Dopamine < 100 pg/ml
It is suggested that each laboratory establish its own normal ranges


Assay Background


Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumors of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). An increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis. The measure­ment of dopamine and its derivatives is of special diagnostic value with children who are suspected to have a neuroblastoma.  The Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA assay kit provides materials for the quantitative measurement of adrenaline, noradrenaline and dopamine in plasma and urine. Noradrenaline, adrenaline and dopamine are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline, N-acyladrenaline and N-acyl-dopamine and then converted enzymatically into N-acylnormeta­nephrine, N-acylmetanephrine and N-acyl-3-methoxytyramine.


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Catecholamine Sensitive Assays: Product Highlights

Additional Information

Assay Principle


The competitive Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine)  ELISA assay kit uses the microtiter plate format. Adrenaline, noradrenaline and dopamine, respectively, are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Typical Standard Curves


Adrenaline, Noradrenaline & Dopamine ELISA Epinephrine Curve
Adrenaline, Noradrenaline & Dopamine ELISA Dopamine Curve
Adrenaline, Noradrenaline & Dopamine ELISA Norepinephrine Curve

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Citations


Egorov, AI;Griffin, SM;Converse, RR;Styles, JN;Sams, EA;Wilson, A;Jackson, LE;Wade, TJ;, (2017). Vegetated land cover near residence is associated with reduced allostatic load and improved biomarkers of neuroendocrine, metabolic and immune functions. Environ. Res.158,508-521.