Adrenaline, Noradrenaline & Dopamine ELISA Assay Kit

$920.00

The Eagle Biosciences Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA Assay Kit is intended for the quantitative determination of adrenaline, noradrenaline, and dopamine in plasma and urine by microplate immunoassay (ELISA). The Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

SKU: AND31-K03 Categories: , ,

Adrenaline, Noradrenaline & Dopamine ELISA Assay kit

For Research Use Only

Size: 3 x 96 wells (1 plate for Adrenaline, 1 plate for Noradrenaline, 1 plate for Dopamine)
Sensitivity:
Urine: Adrenaline: 0.08 ng/ml, Noradrenaline: 0.24 ng/ml, Dopamine 0.44 ng/ml
Plasma: Adrenaline: 5 pg/ml, Noradrenaline: 16 pg/ml, Dopamine 29 pg/ml
Dynamic Range: Adrenaline: 0.5 – 250 ng/ml, Noradrenaline: 1.5 – 500 ng/ml, Dopamine:  1.5 – 2560 ng/ml
Incubation Time:  Overnight
Sample Type: Urine, EDTA Plasma
Sample Size: Urine: 20 µl, Plasma:  300 µl

Controls Included

Product Developed and Manufactured in Germany by DLD Diagnostika EA603/288

Additional Information

Assay Background


The Eagle Biosciences Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA Assay Kit is intended for the quantitative determination of adrenaline, noradrenaline, and dopamine in plasma and urine. The Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.  Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumors of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). An increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis. The measure­ment of dopamine and its derivatives is of special diagnostic value with children who are suspected to have a neuroblastoma.  The Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine) ELISA assay kit provides materials for the quantitative measurement of adrenaline, noradrenaline and dopamine in plasma and urine. Noradrenaline, adrenaline and dopamine are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline, N-acyladrenaline and N-acyl-dopamine and then converted enzymatically into N-acylnormeta­nephrine, N-acylmetanephrine and N-acyl-3-methoxytyramine.

Assay Principle


The competitive Adrenaline, Noradrenaline, & Dopamine (Plasma & Urine)  ELISA assay kit uses the microtiter plate format. Adrenaline, noradrenaline and dopamine, respectively, are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Typical Standard Curves




Manual

Product Manual


Publications

Citations


Egorov, AI;Griffin, SM;Converse, RR;Styles, JN;Sams, EA;Wilson, A;Jackson, LE;Wade, TJ;, (2017). Vegetated land cover near residence is associated with reduced allostatic load and improved biomarkers of neuroendocrine, metabolic and immune functions. Environ. Res.158,508-521.