Noradrenaline ELISA Assay Kit


The Noradrenaline (Norepinephrine) ELISA Assay Kit is intended for the quantitative determination of Noradrenaline (also known as Norepinephrine) in urine or plasma by a microplate enzyme immunoassay (ELISA). The Noradrenaline (Norepinephrine) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.
This product was previously known as NOR31-K01.

Noradrenaline ELISA Assay Kit

Noradrenaline ELISA Assay Kit Developed and Manufactured in Germany by DLD Diagnostika

Size : 1×96 wells
Sensitivity :  Urine: 0.67 ng/ml, Plasma:  45 pg/ml
Dynamic Range : 1.5 –  500 ng/ml (8.9 – 2955 nmol/L)
Incubation Time : Overnight
Sample Type : Urine, Plasma
Sample Size : 20 µL (Urine), 300 µl (Plasma)
Alternative Names: Norepinephrine
For Research Use Only

Controls Included

Assay Principle

The Noradrenaline Assay Kit provides materials for the quantitative measurement of noradrenaline in plasma and urine. Noradrenaline is extracted using a cis-diol-specific affinity gel and acylated to Nacylnoradrenaline and then converted enzymatically into N-acylnormetanephrine. The Noradrenaline ELISA Assay is a competitive ELISA that uses the microtiter plate format that noradrenaline binds to the solid phase of the microtiter plate. Acylated noradrenaline from the sample and solid phase bound noradrenaline compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase noradrenaline is detected by anti-rabbit IgG/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase noradrenaline is inversely proportional to the noradrenaline concentration of the sample.

Related Products

Adrenaline, Noradrenaline & Dopamine ELISA Assay Kit
Noradrenaline (Norepinephrine) Sensitive ELISA
Dopamine Sensitive ELISA Assay

Click the following links to see news and studies using this assay kit
Recent UK Study Utilizes EagleBio’s Adrenaline and Noradrenaline ELISA
EagleBio’s Norepinephrine ELISA Utilized in New Study in Bangladesh

Additional Information

Assay Background

Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitters, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumors of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). Studies show that an increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis.

The Noradrenaline (Norepinephrine) ELISA Assay Kit provides materials for the quantitative measurement of noradrenaline in plasma and urine. Noradrenaline is extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline and then converted enzymatically into N-acylnormeta­nephrine.

Typical Standard Curve

Noradrenaline ELISA Assay Kit


Product Documents



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Using Human Samples

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Using Rat Samples

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Nandi, SS;Zheng, H;Sharma, NM;Shahshahan, HR;Patel, KP;Mishra, PK;, (2016). Lack of miR-133a Decreases Contractility of Diabetic Hearts: A Role for Novel Cross Talk Between Tyrosine Aminotransferase and Tyrosine Hydroxylase. Diabetes, 65(10), 3075-3090.

Zhang, C, Role of beta-endorphin in control of stress and cancer progression in fetal alcohol exposed rats, 2013 

Product Citations