Noradrenaline ELISA Assay Kit
Noradrenaline ELISA Assay Kit Developed and Manufactured in Germany by DLD Diagnostika
Size : 1×96 wells
Sensitivity : Urine: 0.67 ng/ml, Plasma: 45 pg/ml
Dynamic Range : 1.5 – 500 ng/ml (8.9 – 2955 nmol/L)
Incubation Time : Overnight
Sample Type : Urine, Plasma
Sample Size : 20 µL (Urine), 300 µl (Plasma)
Alternative Names: Norepinephrine
For Research Use Only
Controls Included
Assay Principle
The Noradrenaline Assay Kit provides materials for the quantitative measurement of noradrenaline in plasma and urine. Noradrenaline is extracted using a cis-diol-specific affinity gel and acylated to Nacylnoradrenaline and then converted enzymatically into N-acylnormetanephrine. The Noradrenaline ELISA Assay is a competitive ELISA that uses the microtiter plate format that noradrenaline binds to the solid phase of the microtiter plate. Acylated noradrenaline from the sample and solid phase bound noradrenaline compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase noradrenaline is detected by anti-rabbit IgG/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase noradrenaline is inversely proportional to the noradrenaline concentration of the sample.
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Click the following links to see news and studies using this assay kit
Recent UK Study Utilizes EagleBio’s Adrenaline and Noradrenaline ELISA
EagleBio’s Norepinephrine ELISA Utilized in New Study in Bangladesh