MPO Serum / Plasma ELISA
MPO Serum Plasma ELISA Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.65 ng/mL
Dynamic Range: 2.0 – 512 ng/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 100 µL
Alternative Names: Human MPO Serum/Plasma ELISA, Human Myeloperoxidase ELISA
For Research Use Only
Serum/EDTA-Plasma samples from normal healthy adults ages 20 – 60 were collected and measured with this ELISA. Because each of the serum and plasma samples were initially diluted 1:5, the measured value of each sample has to be multiplied by 5 for the true result.
The recommended normal cut-off for serum myeloperoxidase concentration by using this ELISA is 220 ng/mL, and the normal cut off for EDTA-plasma myeloperoxidase concentration is 40 ng/mL. We strongly recommend for each clinical laboratory to establish its own normal cutoff level by measuring normal serum or EDTA-plasma samples with this MPO ELISA.
The MPO Serum Plasma ELISA is designed, developed and produced for the quantitative measurement of human myeloperoxidase in serum and plasma samples. The assay utilizes the two-site “sandwich” technique with selected antibodies that bind to different epitopes of myeloperoxidase.
Assay standards, controls and diluted samples are added directly to wells of a microtiter plate that is coated with antibody to myeloperoxidase. After an incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human myeloperoxidase antibody is added to each well. After the second incubation period, a “sandwich” of solid-phase monoclonal antibody – human myeloperoxidase – HRP conjugated antibody” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and the absorbances are then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human myeloperoxidase in the test sample. A standard curve is generated by plotting the absorbance versus the respective human myeloperoxidase concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human myeloperoxidase in test samples is determined directly from this standard curve.