MPO Stool / Urine ELISA
MPO Stool Urine ELISA Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.3 ng/ml
Dynamic Range: 2.0 – 512 ng/ml
Incubation Time: 2.5 hours
Sample Type: Stool, Urine
Sample Size: 50mg /100 µl
Alternative Names: Myeloperoxidase Stool/Urine ELISA
For Research Use Only
The MPO Stool Urine ELISA is designed, developed and produced for the quantitative measurement of human myeloperoxidase in stool samples. The assay utilizes the two-site “sandwich” technique with selected antibodies that bind to different epitopes of myeloperoxidase.
Assay standards, controls and extracted patient samples are added directly to wells of a microtiter plate that is coated with antibody to myeloperoxidase. After an incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human myeloperoxidase antibody is added to each well. After the second incubation period, a “sandwich” of solid-phase monoclonal antibody – human myeloperoxidase – HRP conjugated antibody” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and the absorbances are then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human myeloperoxidase in the test sample. A standard curve is generated by plotting the absorbance versus the respective human myeloperoxidase concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human myeloperoxidase in test samples is determined directly from this standard curve.
Stool and urine samples from normal healthy adults ages 20 – 60 were collected and measured with this ELISA. With our stool sample collection tube filled with extraction buffer the dilution factor is 1:500. To convert the MPO concentration from nanogram per milliliter to nanogram per gram stool, the following formula should be used:
MPO concentration (ng/mL) X 500 = MPO concentration (ng/g stool)
The recommended normal cut-off for fecal myeloperoxidase concentration by using this ELISA and sample collection system is <2000 ng/g. We strongly recommend for each clinical laboratory to establish its own normal cut-off level by measuring normal stool samples with this ELISA and sample collection system.