MPO Stool Urine ELISA

$595.00

The Myeloperoxidase (MPO) ELISA Assay kit is intended for use in the quantitative determination of human myeloperoxidase (MPO) levels in stool and urine samples. The Eagle Biosciences Myeloperoxidase (MPO) ELISA Assay kit is useful for detecting elevated levels of myeloperoxidase in stool samples, which may serve as a sensitive predictor for inflammatory activities in the gastrointestinal tract. The Eagle Biosciences MPO ELISA Assay kit is for research use only and not to be used in diagnostic procedures.

SKU: MPO39-K01 Categories: , ,

MPO Stool / Urine ELISA

MPO Stool Urine ELISA Developed and Manufactured in the USA

Size: 1×96 wells
Sensitivity: 0.3 ng/ml
Dynamic Range: 2.0 – 512 ng/ml
Incubation Time: 2.5 hours
Sample Type: Stool, Urine
Sample Size: 50mg /100 µl
Alternative Names: Myeloperoxidase Stool/Urine ELISA
For Research Use Only

Controls Included


Assay Principle

The MPO Stool Urine ELISA is designed, developed and produced for the quantitative measurement of human myeloperoxidase in stool samples. The assay utilizes the two-site “sandwich” technique with selected antibodies that bind to different epitopes of myeloperoxidase.

Assay standards, controls and extracted patient samples are added directly to wells of a microtiter plate that is coated with antibody to myeloperoxidase. After an incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human myeloperoxidase antibody is added to each well. After the second incubation period, a “sandwich” of solid-phase monoclonal antibody – human myeloperoxidase – HRP conjugated antibody” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and the absorbances are then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human myeloperoxidase in the test sample. A standard curve is generated by plotting the absorbance versus the respective human myeloperoxidase concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human myeloperoxidase in test samples is determined directly from this standard curve.


Expected Values
Stool and urine samples from normal healthy adults ages 20 – 60 were collected and measured with this ELISA. With our stool sample collection tube filled with extraction buffer the dilution factor is 1:500. To convert the MPO concentration from nanogram per milliliter to nanogram per gram stool, the following formula should be used:
MPO concentration (ng/mL) X 500 = MPO concentration (ng/g stool)

The recommended normal cut-off for fecal myeloperoxidase concentration by using this ELISA and sample collection system is <2000 ng/g. We strongly recommend for each clinical laboratory to establish its own normal cut-off level by measuring normal stool samples with this ELISA and sample collection system.


Related Products

(MPO) Serum / Plasma ELISA Assay Kit
Anti-MPO ELISA
Mouse MPO ELISA Kit

Additional Information

Assay Procedure


  1. Add 100 µl of Standards, Controls and extracted patient samples into the designated microwells.
  2. Seal the plate wells securely, cover with foil or other material to protect from light, and rotate on an ELISA plate shaker (small orbit radius) for 1.5 hr. ± 5 minutes at 400 to 450 rpm.
  3. Just prior to the end of the incubation time, dilute the proper amount of Tracer Antibody for the assay.
  4. Wash each well 5 times by dispensing 350 µL of working wash solution into each well and then completely aspirating the contents. Alternatively, an automated microplate washer can be used.
  5. Add 100 µL of above Tracer Antibody to each well.
  6. Seal the plate wells securely, cover with foil or other material to protect from light, and rotate on an ELISA plate shaker (small orbit radius) for 45 minutes ± 5 minutes at 400 to 450 rpm.
  7. Wash each well 5 times by dispensing 350 µL of working wash solution into each well and then completely aspirating the contents. Alternatively, an automated microplate washer can be used.
  8. Add 100 µL of ELISA HRP Substrate into each of the wells.
  9. Cover the plate with aluminum foil to or other material to avoid exposure to light. Incubate plate static, at room temperature for 20 minutes.
  10. Immediately add 100 µL of ELISA Stop Solution into each of the wells. Mix gently.
  11. Read the absorbance at 405 nm with reference filter at 620 nm or 650 nm.

Typical Standard Curve


Myeloperoxidase (MPO) Stool / Urine ELISA

Documents

Product Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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