Mouse AT1R IgG Antibody ELISA

$1,765.00

This Mouse AT1R IgG Antibody ELISA is designed for the determination of mouse antibodies (IgG) against the Angiotensin II receptor subtype I in serum and plasma.  The Eagle Biosciences Anti Angiotensin Receptor 1 (ATR1) IgG Antibody ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Mouse AT1R IgG Antibody ELISA

The Mouse AT1R IgG Antibody ELISA is For Research Use Only

Sensitivity: 1.25 U/ml
Dynamic Range: 1.25 – 40 U/ml
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma
Sample Size: 100 µL
Alternative Name: Mouse Anti Angiotensin II Receptor 1 IgG Antibody ELISA Assay Kit


Sample Preparation and Storage
Dilute the samples with diluent using 1:100 dilution (eg. 5 µl serum or plasma + 495 µl diluent). If samples generate values outside the standard curve, the dilution factor may be quite varied. Store the undiluted samples at room temperature for 48 hours, 2-8°C 4- days, and long-term storage for up to 12 months at –20 °C. Avoid repeated freeze-thaw cycles.


Assay Background

Angiotensin II is a peptide hormone which causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It occupies an important role in the Renin-Angiotensin-System (RAS). Angiotensin II mediates the effects through G-Protein-coupled receptors, the Angiotensin receptors. The occurrence of autoantibodies against AT1 receptor is associated with an increased risk of an immunologic rejection after organ transplantation. The presence of AT1 autoantibodies correlate with the existence and course of Scleroderma.
This Mouse Anti Angiotensin Receptor 1 (ATR1) IgG Antibody ELISA Assay Kit is designed for the determination of mouse antibodies (IgG) against the Angiotensin II receptor subtype I in serum and plasma.


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Rat AT1R IgG Antibody ELISA

Additional Information

Assay Principle


The Eagle Bioscience’s Mouse Anti Angiotensin Receptor 1 IgG Antibody ELISA Assay Kit is an antibody screening test. Angiotensin II Receptor has been pre-coated onto a microtiter plate. During the first incubation the rat anti-Angiotensin II receptor 1-IgG-Antibodies of the samples are immobilized on the plate. The autoantibodies are detected with a POD labelled anti-mouse IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-Angiotensin II receptor 1-antibody (IgG).

Assay Procedure


  1. Prepare all reagents and samples as directed in the previous section.
  2. Pipette 100 µl of diluted samples, standards, controls or diluent (as blank) into the wells.
  3. Seal wells with adhesive strip and incubate for 2 hours at 2-8°C temperature.
  4. Aspirate fluid from wells and wash three times with 300 µl wash buffer.
  5. After the last wash, invert the plate and tap on a clean paper towel.
  6. Dispense 100 μl of diluted HRP conjugate into each well.
  7. Seal wells with adhesive strip and incubate for 1 hour (with shaking) at room temperature.
  8. Repeat the wash as in step 4.
  9. Dispense 100 µl of TMB substrate solution into each well.
  10. Incubate for 20 minutes at room temperature in the dark.
  11. Add 100 µl of stop solution to each well.
  12. Determine the absorbance within 30 minutes at 450 nm. A reference wavelength of 620 nm/690 nm is recommended.

Manual

Product Manual


Publications

References


  1. Reinsmoen NL, Lai C-H, Heidecke H, Haas M, Cao K, Ong G, Naim M, Wang Q, Mirocha J, Kahwaji J, Vo AA, Jordan SC, and Dragun D:  Anti-Angiotensin Type 1 Receptor Antibodies Associated With Antibody Mediated Rejection in Donor HLA Antibody Negative Patients.  Transplantation 2010;90: 1473–1477
  2. Kelsch R, Everding AS, Kuwertz-Bröking E, Brand E, Spriewald BM, Sibrowski W, Konrad M, Dragun D:  Accelerated Kidney Transplant Rejection and Hypertensive Encephalopathy in a Pediatric Patient Associated With Antibodies Against Angiotensin Type 1 Receptor and HLA Class II.  Transplantation 2011 Nov 27;92(10):e57-9