Mouse/Rat C-Peptide ELISA Assay Kit
The Mouse/Rat C-Peptide ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.3ng/mL
Standard Range: 0.3ng/mL-6ng/mL
Incubation Time: 1 hour 45 minutes
Sample Type: Serum and plasma
Sample Size: 10 µl
Assay Principle
The Mouse/Rat C-Peptide assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against C-peptide. Standards and samples are added into the wells and co-incubated with a biotin labeled monoclonal antibody. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, TMB substrate is added and color develops in proportion to the amount of C-peptide bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured C-peptide, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Sample Handling
If a sample has a C-peptide level greater than the highest standard, the
sample should be diluted with 0 ng/ml C-peptide standard solution and
the assay should be repeated.
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