Insulin ELISA Assay Kit
Insulin ELISA Assay Kit Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.1817 mU/L (1.09 pmol/L)
Dynamic Rang: 2.6 mU/L – 138 mU/L (15.6 – 828 pmol/L)
Incubation Time: 2.0 hours
Sample Type: Serum, Plasma
Sample Size: 25 µL
For Research Use Only
1 µg/L = 23 mU/L
1 mU/L = 6.0 pmol/L
This Insulin ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human insulin in serum and /or EDTA-plasma samples. The assay utilizes the “sandwich” technique with selected antibodies that bind to various epitopes of Insulin.
Assay standards, controls and patient samples are added directly to wells of a microplate that is coated with an anti-human Insulin specific antibody. Simultaneously, a horseradish peroxidase-conjugated monoclonal Insulin specific antibody is added to each well. After the first incubation period, the antibody on the wall of microtiter well captures human Insulin in the sample and unbound proteins in each microtiter well are washed away. A “sandwich” of “anti-Insulin antibody — human Insulin — HRP conjugated tracer antibody” is formed. The unbound tracer antibody is removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to human Insulin on the wall of the microtiter well is directly proportional to the amount of Insulin in the sample. A standard curve is generated by plotting the absorbance versus the respective human Insulin concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of human Insulin in test samples is determined directly from this standard curve.