Insulin ELISA Assay Kit

$375.00

The Insulin ELISA Assay Kit is intended for use in the quantitative determination of human insulin in serum and/or plasma.The Eagle Biosciences Insulin ELISA Assay kit is intended for research use only and not intended for diagnostic procedures.

SKU: INS31-K01 Categories: , ,

Insulin ELISA Assay Kit

Insulin ELISA Assay Kit Developed and Manufactured in the USA

Size:  1×96 wells
Sensitivity:  0.1817 mU/L (1.09 pmol/L)
Dynamic Rang:  2.6 mU/L – 138 mU/L  (15.6 – 828 pmol/L)
Incubation Time:  2.0 hours
Sample Type:  Serum, Plasma
Sample Size: 25 µL
For Research Use Only

Conversion Factor
1 µg/L = 23 mU/L
1 mU/L = 6.0 pmol/L


Assay Principle

This Insulin ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human insulin in serum and /or EDTA-plasma samples. The assay utilizes the “sandwich” technique with selected antibodies that bind to various epitopes of Insulin.

Assay standards, controls and patient samples are added directly to wells of a microplate that is coated with an anti-human Insulin specific antibody. Simultaneously, a horseradish peroxidase-conjugated monoclonal Insulin specific antibody is added to each well. After the first incubation period, the antibody on the wall of microtiter well captures human Insulin in the sample and unbound proteins in each microtiter well are washed away. A “sandwich” of “anti-Insulin antibody — human Insulin — HRP conjugated tracer antibody” is formed. The unbound tracer antibody is removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to human Insulin on the wall of the microtiter well is directly proportional to the amount of Insulin in the sample. A standard curve is generated by plotting the absorbance versus the respective human Insulin concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of human Insulin in test samples is determined directly from this standard curve.


Products Related to Insulin ELISA Assay

Insulin Ultrasensitive ELISA Assay Kit
Human Insulin Biotinylated (Biotin-Insulin)
IAA (Insulin Autoantibodies) ELISA Assay Kit

Additional Information

Assay Background


Insulin is a peptide hormone, produced by beta cells of the pancreas. Enzymatic cleavage of proinsulin results in the production of equimolar amounts of insulin and C-peptide in circulation. Insulin is central to regulating carbohydrate and fat metabolism in the body.  Excessive amounts of insulin are associated with excess amounts of glucose in the system. High levels of insulin are known to cause weight gain, water bloating, high blood pressure, magnesium deficiency and an increase in the amount of inflammatory compounds in the blood, which causes blood clots and blood vessel damage.

Insulin, when insufficiently produced, results in diabetes mellitus. In most cases, a high fasting insulin level is consistent with insulin resistance symptoms, but in some cases, it can be caused by more serious conditions such as Cushing’s syndrome, acromegaly or possibly insulinoma.

Typical Standard Curve


Insulin ELISA Assay Standard Curve

Manual

Product Manual