Fatty Acid Binding Protein 4 (FABP4) ELISA Assay Kit

$640.00

The FABP-4 ELISA Assay Kit is designed for quantification of human FABP4 in serum, plasma, and adipocyte extracts or cell culture media samples. The Eagle Biosciences FABP-4 ELISA Assay Kit is for research use only and not to be used for diagnostic purposes.

Fatty Acid Binding Protein 4 (FABP4) ELISA Assay Kit

The Fatty Acid Binding Protein 4 (FABP4) ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.39ng/mL
Standard Range: 0.39g/mL-25ng/mL
Incubation Time: 2 hours 35 minutes
Sample Type: Serum, plasma, and cell culture supernates
Sample Size: 100 µl
Alternative Names: Fatty-acid binding protein 4,A-FABP, aP2


Assay Principle

The Fatty Acid Binding Protein 4 assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a mouse monoclonal antibody specific for human FABP4. Standards and samples are pipetted into the wells and any Human FABP4 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FABP4 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate is added, after the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of human FABP4 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FABP4, the unknown sample concentration can be interpolated from a reference curve included in each assay.


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Additional Information

Assay Background


Fatty-acid binding protein 4 (FABP4), also termed adipocyte fatty-acid binding protein (A-FABP), or aP2, is a novel adipocyte-expressed factor which accounted for ~6% of total cellular proteins. Several animal experiments suggested that FABP-4 plays a key role in the link between obesity and various features of metabolic syndrome. Mice with targeted disruption of FABP-4 accompany FABP-5 almost completely protect against diet-induced obesity, insulin resistance, dyslipidemia, type 2 diabetes, and fatty liver disease. Studies in human found FABP-4 serum levels were significantly increased in overweight and obese subjects, which predicted the risk to develop a metabolic syndrome and type 2 diabetes. Additionally, serum FABP-4 levels were associated with nonalcoholic fatty liver disease, carotid atherosclerosis and coronary artery disease.

Typical Standard Curve


FABP-4 ELISA Kit

Documents

Product Manual