Mouse Monoclonal Anti-PCNA Antibody Clone Р60
The Mouse Monoclonal Anti-PCNA Antibody Clone Р60 is For Research Use Only
Immunogen: Full Size Recombinant Protein
Alternative Names: Proliferating Cell Nuclear Antigen
Host: Mouse
Class: IgG1, kappa
Specificity: Human
Application: IHC on FFPE (formalin fixed paraffin embedded tissue sections)
Positive Control: Tonsil Tissue, Colon Adenocarcinoma
Demasking: Temperature, citrate buffer, pH 6 (12-310002)
Staining: Nuclear
Reagent provided: This antibody is immunoglobulin IgG2a diluted in 10 mM Phosphate buffered saline (PBS), pH 7.4 containing 1% bovine serum albumin (BSA) and 0.05% ProClin™ 300 as antimicrobial agent.
Usage dilution:
10-310022-7: Ready to Use
10-310022-01; 10-310022-05; 10-310022-1: Dilute 1:50 to 1:100 with Antibody Diluent (REF 12-310001) before use. Optimum dilution factor may vary depending on the specimen and preparation process and should be determined by each individual investigator.
Epitope retrieval: Staining of formalin fixed, paraffin embedded tissue sections is significantly enhanced by pretreatment with Citrate Buffer, pH 6 (REF 12-310002).
Staining procedure: Incubate this antibody with tissue section for 30-60 minutes at room temperature. Follow the instructions from the selected detection system.
Storage: Store at 2-8°C. Do not use after expiration date.
Background: Proliferating Cell Nuclear Antigen (PCNA) is actively expressed by cells undergoing the division cycle. Antibodies stain actively proliferating tissues.
Product Developed and Manufactured by Diagomix.
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