Cancer Biomarker ELISA Assay

Mouse EGFR ELISA Kit

$585.00

The Mouse EGFR ELISA Kit is intended for the quantitation of Mouse EGFR concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Mouse EGFR ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: MFR11-K01 Categories: , ,

Mouse EGFR ELISA Kit

The Mouse EGFR ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <15pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Names: Epidermal Growth Factor Receptor, ErbB-1

Assay Principle

The Mouse Egfr Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Egfr with a 96-well strip plate that is pre-coated with antibody specific for Egfr. The detection antibody is a biotinylated antibody specific for Egfr. The kit contains recombinant Mouse Egfr with immunogen: Expression system for standard: NSO; Immunogen sequence: L25-S647. The kit is analytically validated with ready to use reagents. To measure Mouse Egfr, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Egfr in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse Egfr in the sample.

Related Products

Human EGFR ELISA Kit
Mouse VEGFR2/KDR ELISA Kit
Mouse FLT4/VEGFR3 ELISA Kit

Additional Information

Assay Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Typical Standard Curve

Mouse EGFR ELISA Kit

Documents

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