Human EGFR ELISA Kit

$415.00

The Human EGFR ELISA Kit is intended for the quantitation of Human EGFR concentrations in cell culture supernates, serum, plasma(heparin, EDTA) and human milk. The Eagle Biosciences Human EGFR ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: EGR31-K01 Categories: , ,

Human EGFR ELISA Kit

The Human EGFR ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <1pg/ml
Standard Range: 156pg/ml-10,000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, Serum, Plasma (heparin, EDTA) and Human milk.
Sample Size: 100 µl
Alternative Name: Epidermal Growth Factor Receptor


Assay Background

The epidermal growth factor receptor(EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family(EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR(ErbB-1), HER2/c-neu(ErbB-2), Her 3(ErbB-3) and Her 4(ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.


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Additional Information

Assay Principle


The Human EGFR Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human EGFR with a 96-well strip plate that is pre-coated with antibody specific for EGFR. The detection antibody is a biotinylated antibody specific for EGFR. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human EGFR with immunogen: Expression system for standard: NSO; Immunogen sequence: M1-S645. The kit is analytically validated with ready to use reagents. To measure Human EGFR, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human EGFR in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human EGFR in the sample.

Typical Standard Curve


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