Human FGF19 ELISA Kit

$465.00

The Human FGF19 ELISA Kit is intended for the quantitation of Human FGF19 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human FGF19 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: FG931-K01 Categories: , ,

Human FGF19 ELISA Kit

The Human FGF19 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 15.6pg/ml-1000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, Cell lysates, Serum and Plasma
Sample Size: 100 µl
Alternative Names: Fibroblast growth factor 19


Assay Principle

The Human FGF19 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FGF19 with a 96-well strip plate that is pre-coated with antibody specific for FGF19. The detection antibody is a biotinylated antibody specific for FGF19. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human FGF19 with immunogen: Expression system for standard: E.coli; Immunogen sequence: L25-K216. The kit is analytically validated with ready to use reagents. To measure Human FGF19, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FGF19 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human FGF19 in the sample.


Related Products

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Additional Information

Assay Background


FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains a signal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity.

Typical Standard Curve


Human FGF19 ELISA Kit

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