Monoclonal Mouse Anti-Human MLH1 Antibody

$80.00$270.00

The Eagle Biosciences Monoclonal Mouse Anti-Human MLH1 antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. The Monoclonal Mouse Anti-Human MLH1 Antibody is for research use only and not to be used for diagnostic procedures.

SKU: IHC409 Categories: ,

Monoclonal Mouse Anti-Human MLH1 Antibody

The Monoclonal Mouse Anti-Human MLH1 Antibody is For Research Use Only

Clone: IHC409
Source: Mouse Monoclonal
Positive Control: Colon, Colon Carcinoma
Localization: Nuclear


Background

MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumours that are microsatellite-unstable.

Antibody Principle

Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.


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Documents

Product Manual


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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