Monoclonal Rabbit Anti-Human Estrogen Receptor Antibody
The Monoclonal Rabbit Anti-Human Estrogen Receptor Antibody is For Research Use Only
Source: Rabbit Monoclonal
Positive Control: Breast Carcinoma
Estrogen Receptors (ER) are a group of nuclear hormone receptors activated by the hormone estrogen. ER is found in normal epithelial cells of the breast and endometrium, as well as in breast cancer cells.
Visualization of the antigen present in tissue sections is accomplished in a multistep immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
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