Monoclonal Mouse Anti-Human BRAF V600E Antibody


The Eagle Biosciences Monoclonal Mouse Anti-Human BRAF V600E antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. The Monoclonal Mouse Anti-Human BRAF V600E Antibody is for research use only and not to be used for diagnostic purposes.

SKU: IHC600 Categories: ,

Monoclonal Mouse Anti-Human BRAF V600E Antibody

The Monoclonal Mouse Anti-Human BRAF V600E Antibody is For Research Use Only

Clone: IHC600
Source: Mouse Monoclonal
Positive Control: Colorectal Adenocarcinoma/Thyroid Papillary Carcinoma with the BRAF V600E Mutation
Localization: Cytoplasmic.


Serine/Threonine-Protein Kinase B-Raf (BRAF) is a cytoplasmic serine-threonine kinase of the RAF family, which mediates downstream cellular responses to growth signals through the mitogen-activated protein kinase (MAPK) signaling pathway. Oncogenic mutations in the BRAF gene, 80% of which are a single V600E substitution within the kinase domain, constitutively activate the MAPK signaling pathway and result in increased cell proliferation and apoptosis resistance. The V600E mutation is observed in colorectal cancer, non-Hodgkin’s lymphoma, papillary thyroid carcinoma, malignant melanoma, non-small-cell lung carcinoma, and lung adenocarcinoma. BRAF V600E is therefore an important immunohistochemical marker for tumor diagnosis and prognosis.

Antibody Principle

Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.

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Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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