Monoclonal Mouse Anti-Human MDM2 Antibody

$270.00

The Eagle Biosciences Monoclonal Mouse Anti-Human MDM2 antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. The Monoclonal Mouse Anti-Human MDM2 Antibody is for research use only and not to be used for diagnostic procedures.

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SKU: IHC620 Categories: ,

Monoclonal Mouse Anti-Human MDM2 Antibody

The Monoclonal Mouse Anti-Human MDM2 Antibody is For Research Use Only

Clone: IHC620
Source: Mouse Monoclonal
Positive Control: Liposarcoma
Localization: Nuclear


Background

Mouse Double Minute 2 Homolog (MDM2), also known as HDM2 in humans, is crucial in negative regulation of the p53 tumour suppressor. Negative regulation is mediated through both the ubiquitination of p53/TP53, as well as inhibition of p53 transcriptional activation. Reports have indicated an overexpression of MDM2 to be associated with a number of different human tumour types, including soft tissue sarcomas, osteosarcomas, and breast tumours. When co-overexpressed with the CDK4 protein, MDM2 can also aid in the detection of well-differentiated liposarcomas and de-differentiated liposarcoma.

Antibody Principle

Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope


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Documents

Product Manual


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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