FSH ELISA Assay Kit

$230.00

The Eagle Biosciences FSH ELISA Assay Kit is a direct solid phase immunoassay for the quantitative determination of Follicle-Stimulating Hormone (FSH) in human serum or plasma. FSH ELISA Assay Kit is intended for research use only and not to be used in diagnostic procedures.

SKU: DCM010 Categories: , ,

FSH ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.17 mIU/ml
Dynamic Range: 5 -100 mIU/ml
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 50 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Follicle Stimulating hormone (FSH) is a glycoprotein consisting of two subunits with an approximate molecular mass of 35,500 daltons. The α-subunit is similar to other pituitary hormones [luteinizing stimulating hormone (LH), thyroid stimulating hormone (TSH) and chorionic gonadotropin (hCG)] while the β-subunit is unique. The β- subunit confers the biological activity to the molecule. Stimulation by gonadotropin-releasing hormone (GnRH) causes release of FSH, as well as LH, from the pituitary and is transported by the blood to their sites of action, the testes or ovary.

In men, FSH acts on the Sertoli cells of the testis, stimulating the synthesis of inhibin, which appears to specifically inhibit further FSH secretion, and androgen-binding protein. Thus, it indirectly supports spermatogenesis. In women, FSH acts on the granulosa cells of the ovary, stimulatin  steroidogensis. All ovulatory menstrual cycles have a characteristic pattern of FSH, as well as LH, secretion. The menstrual cycle is divided into a follicular phase and a luteal phase by the midcycle surge of the gonadotropins (LH and FSH). As the follicular phase progresses, FSH concentration decreases. Near the time ovulation occur, about midcycle, FSH peaks (lesser in magnitude than LH) to its highest level. The clinical usefulness of the measurement of Follicle Stimulating hormone (FSH) in ascertaining the homeostasis of fertility regulation via the hypothalamic – pituitary – gonadal axis has been well established (1,2).

Assay Principle

In the FSH ELISA Assay Kit, the essential reagents required for an immunoenzymatic assay include high affinity and specificity antibodies (enzyme-linked and immobilised) with different and distinct epitope recognition, in excess, and native antigen.

In this procedure the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti FSH antibody.

Upon mixing monoclonal biotinylated antibody, the enzyme labeled antibody and a serum containing the native antigen, reation results between the native antigen and the antibodies without competition or steric hindrance to form a soluble sandwich complex.

After equilibrium is attained, the antibody-bound fraction is separated from unbound antigen by a washing step.

The enzyme activity in the antibody-bound fraction is directly proportional to the native antigen concentration. By using several different serum references of known antigen values, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.

Manual

Product Manual