Prolactin ELISA Assay Kit

$190.00

The Eagle Biosciences Prolactin ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of prolactin (PRL) in human serum. The Prolactin ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: PRL31-K01 Categories: , ,

Prolactin ELISA Assay Kit

The Prolactin ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 10 μIU/mL
Dynamic Range: 20 – 3200 μIU/mL
Incubation Time: 1 hour 15 minutes
Sample Type: Serum
Sample Size: 25 µL


Assay Background

Prolactin (PRL) is a polypeptide hormone synthesized by the lactotropic cells of the anterior pituitary gland. Structurally, it is similar to two other polypeptide hormones namely, growth hormone, and placental lactogen. PRL is a polypeptide containing 199 amino acids, while growth hormone and placental lactogen each have 191 amino acids. There is approximately 100ug of prolactin in the human pituitary gland, which is a very small amount when compared to growth hormone, which is present at 8-10 mg. The target organ of prolactin is the breast (mammary gland). Its main physiological action is not only to initiate but also to sustain lactation. The hypothalamus secretes dopamine, which has a direct effect of inhibition of the secretion of PRL. If dopamine is not available or absent the secretion of PR: is autonomous. Clinical trends:

  • In patients where tumors secrete prolactin there is a remarkable increase in the PRL level, which then decreases the secretion of gonadotropin resulting in infertility.
  • If the pituitary gland is deficient it leads to failure of lactation.
  • In Sheehan’s syndrome the pituitary gland is deficient, therefore the PRL level is reduced.
  • A few conditions where increases in prolactin levels are found include: hyperprolactinemia, adnomas of the pituitary gland, sleep, pregnancy, hypothyroidism, prolactinomas and stress.
  • Prolactinomas are pituitary tumors secreting prolactin, found most frequently in females. In females they lead to amenorrhoea, which could be primary or secondary and give rise to a decrease in gonadotropin secretion by the pituitary. In men some degree of impotency accompanied by a low testosterone level occurs, followed by azospermia.

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Additional Information

Assay Principle


The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specifi c for prolactin is immobilized onto the microplate and another monoclonal antibody specifi c for a different region of prolactin is conjugated to horse radish peroxidase (HRP). Prolactin from the sample and standards are allowed to bind simultaneously to the plate and to the HRP conjugate. The washing and decanting steps remove any unbound HRP conjugate. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the con-centration of prolactin in the sample. A set of standards is used to plot a standard curve from which the amount of prolactin in patient samples and controls can be directly read.

Standard Curve

Documents

Product Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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