Intact PTH ELISA Assay
Intact PTH ELISA Assay Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.77 pg/mL
Dynamic Range: 15 – 1215 pg/mL
Incubation Time: 3.5 hours
Sample Type: EDTA Plasma
Sample Size:200 uL
Alternative Names: Parathyroid hormone
For Research Use Only
Assay Background for Intact PTH ELISA Assay
Parathyroid hormone PTH is a 84 amino acid polypeptide with an approximate molecular weight of 9500 Dalton. PTH is the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluid. This hormone is secreted from cells of the parathyroid glands and finds its major target cells in bone and kidney.
This Human Intact PTH ELISA Assay kit is designed, developed and produced for the quantitative measurement of human PTH in EDTA-plasma sample. The assay utilizes the two-site “sandwich” technique with selected antibodies that bind to N-terminal and mid-region epitopes of PTH.
Assay calibrators, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to the N-terminal of human PTH. After the first incubation period, unbound material in the sample is removed in subsequent washing step.
A horseradish peroxidase (HRP) conjugated anti mid-region of human PTH antibody is added to each well. After the second incubation period, a “sandwich” of solid-phase polyclonal antibody – human PTH – HRP conjugated monoclonal antibody” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (i.e. ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human PTH in the test sample. A calibration curve is generated by plotting the absorbance versus the respective human PTH concentration for each calibrator on a point-to-point or 4-parameter curve fitting. The concentration of human PTH in test samples is determined directly from this calibration curve.