iLite IL-2 Assay Ready Cells

$1,190.00

The iLite IL-2 Assay Ready Cells can be used for the quantification of IL-2 activity, or neutralizing antibody response against IL-2 in test samples, including human serum. The iLite IL-2 Assay Ready Cells are for research use only.

SKU: BM4002 Category:

iLite IL-2 Assay Ready Cells

iLite IL-2 Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite IL-2 Assay Ready Cells are intended for testing:

  • Quantification of functional IL-2
  • Quantification of IL-2 inhibitor

Content: >250 μL vial of Assay Ready Cells suspended in cryoprotective medium from Amsbio
Related Names: Interleukin-2 Assay Ready Cells
Receipt and Storage: Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing and should be diluted immediately after thawing.
For Research Use Only


Key benefits of iLite® Assays

  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (>10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects

Product Description

iLite® IL-2 ARCs are avian DT-40 cells which have been genetically engineered and optimized to be responsive to IL-2, resulting in a proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.


Products Related to IL-2 Assay Ready Cells

Human IL-2 High Sensitivity ELISA Assay Kit
Human IL-2 ELISA Assay Kit
iLite IL-23 Assay Ready Cells

Additional Information

Background


Interleukin-2 (IL-2) is part of the body’s natural response to microbial infection and a tool in the immune system discrimination between foreign (“non-self”) and “self”. It signals through the IL-2 receptor, a complex consisting of three chains, termed alpha, beta, and gamma. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily as a stimulant of T- and B-cell growth and maturation. IL-2 activates and stimulates the growth of immune cells, most importantly T-Cells, but also Natural Killer Cells (NK Cells), both can eliminate cancer cells directly. Anti-tumor effects of IL-2 appear to be mediated by its effects on NK Cells, Lymphokine-activated Killer Cells and other cytotoxic cells (1,2).

Frequently Asked Questions


Manual

Product Manual


Publications

References


1. Liao W, Lin JX, Leonard WJ (October 2011). “IL-2 family cytokines: new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation”. Current Opinion in Immunology. 23 (5): 598–604.
2. Gaffen SL, Liu KD (November 2004). “Overview of interleukin-2 function, production and clinical applications”. Cytokine. 28 (3): 109–23.