Nivolumab ELISA mAb-based Assay

$1,310.00

The Nivolumab ELISA mAb-based Assay is an enzyme immunoassay for the specific determination of free Nivolumab in serum and plasma. The Eagle Biosciences Nivolumab (Opdivo®) ELISA (mAb-based) Assay Kit is for research use only and is not intended for diagnostic or therapeutic procedures.

Nivolumab ELISA mAb-based Assay

The Nivolumab ELISA mAb-based Assay is For Research Use Only

Size: 1 x 96 wells
Sensitivity: 20 ng/mL
Dynamic Range: 20 -600 ng/ml
Incubation Time: 2 hours
Sample Type:  Serum, Plasma
Sample Size: 10 µL
Alternative Name: Opdivo


Assay Principle

This Nivolumab (mAb-based) ELISA is based on Nivolumab-specific mouse monoclonal antibody (catcher Ab, ImmunoGuide clone NY-11C3). Diluted standards and samples are incubated in the microtiter plate coated with IG-NY-11C3 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG antibody is added and binds to the Fc part of Nivolumab. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Nivolumab in the sample or standard. Results of samples can be determined by using the standard curve.


Storage an Stability of the Kit
The Nivolumab (mAb-based) ELISA is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C. The usual precautions for venipuncture should be observed. It is important to preserve the chemical integrity of a blood specimen from the moment it is collected until it is assayed. Do not use grossly hemolytic, icteric or grossly lipemic specimens. Samples appearing turbid should be centrifuged before testing to remove any particulate material.


Related Products

Nivolumab (Opdivo) Antibodies ELISA Kit
Ustekinumab mAb-based ELISA Assay
Ranibizumab mAb-based Assay Kit

Additional Information

Assay Background


The Eagle Biosciences Nivolumab (Opdivo®) ELISA (mAb-based) Assay Kit is an enzyme immunoassay for the specific determination of free Nivolumab in serum and plasma. The Eagle Biosciences Nivolumab (Opdivo®) ELISA (mAb-based) Assay Kit is for research use only and is not intended for diagnostic or therapeutic procedures.

Assay Procedure


  1. Pipette 100µL of Assay Buffer into each of the wells to be used.
  2. Pipette 50 µL of each 1:10 Diluted Standard, and 1:200 Diluted Samples into the respective wells of the microtiter plate.
  3. Cover the plate with adhesive seal. Shake plate carefully. Incubate 60 min at room temperature (RT, 20-25°C).
  4. Remove adhesive seal. Aspirate or decant the incubation solution. Wash the plate 3 X 300 μL of Diluted Wash Buffer per well. Remove excess solution by tapping the inverted plate on a paper towel.
  5. Pipette 100 μL of Enzyme Conjugate (HRP-anti human IgG) into each well.
  6. Cover plate with adhesive seal. Shake plate carefully. Incubate 30 min at RT.
  7. Remove adhesive seal. Aspirate or decant the incubation solution. Wash the plate 3 X 300 μL of Diluted Wash Buffer per well. Remove excess solution by tapping the inverted plate on a paper towel.
  8. Pipette 100 µL of Ready-to-Use TMB Substrate Solution into each well.
  9. Incubate 10 min at RT. Avoid exposure to direct sunlight.
  10. Stop the substrate reaction by adding 100 µL of Stop Solution into each well. Briefly mix contents by gently shaking the plate. Color changes from blue to yellow.
  11. Measure optical density (OD) with a photometer at 450 nm (Reference at OD620 nm is optional) within 15 min after pipetting the Stop Solution.

Typical Standard Curve


Nivolumab ELISA mAb-based Assay

Documents

Product Manual


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations