iLite FGF-21 Assay Ready Cells

$1,240.00

The Eagle Biosciences iLite assay ready cells are designed for the specific detection of drug potency in serum/plasma as well as neutralizing antibodies. They utilize an assay technique called reporter gene assay ( RPG ) to analyze serum/plasma samples. The reporter genes utilized are encoded with bioluminescent luciferase (Firefly luciferase). Different levels of luminescence detected from each reporter gene cell indicates different levels of expression. The iLite FGF-21 Assay Ready Cells are for research use only and not for use in diagnostic procedures.

iLite FGF-21 Assay Ready Cells

For Research Use Only

The Eagle Biosciences iLite® FGF-21 Assay Ready Cells are intended for :

  • Testing FGF-21 functional activity
  • Testing NAb against FGF-21

Key benefits of iLite® Assays

  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (>10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects

Product Developed and Manufactured by Svar Life Science

Additional Information

Background

Human fibroblast growth factor 21 (FGF21) is a member of a family of the atypical fibroblast growth factors that include FGF19 and FGF23 in man. FGF21 lacks the heparin-binding domain of conventional FGFs and can consequently diffuse throughout the body and function as a hormone. FGF21 stimulates glucose uptake in adipocytes and the effects on glucose uptake is additive with insulin (1). The iLite® FGF21 Assay Ready Cells are a genetically engineered reporter gene cell line responsive to FGF21 by specific and proportional expression of Firefly Luciferase. The receptor chain FGFR1c is overexpressed on the surface together with the co-factor β-Klotho which has been genetically optimized to enhance sensitivity. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

Assay Priniciple

The iLite® FGF21 Assay Ready Cells are engineered cells optimized to express Firefly luciferase under the control of an FGF21 responsive promoter. Binding of FGF21 to the hetero-dimeric cell surface receptor composed of the tyrosine kinase FGFR1c receptor and β-Klotho results in activation of the FGF21 regulated Firefly luciferase reporter gene construct. The Firefly luciferase signal can be measured in a luminometer following addition and incubation with a luciferase substrate. The Firefly luciferase signal is proportional to the functional activity of FGF21 in the sample.

Assay Quick Guide

Click here for the Assay Summary: FGF-21 Drug Activity Protocol

Why Choose the iLite® FGF-21 Assay Ready Cells?

  • iLite technology employs the cells’ inherent signaling pathways – a true model of cellular events
  • Assay Ready Cells – no culturing required!
  • Highly reproducible between batches
  • Normalization gene allows for consistent results
  • iLite® FGF21 Assay Ready Cells are based on the human embryonic kidney cell line, HEK293 (ATCC# CRL-1573), and have been genetically engineered and optimized to be responsive to human FGF21 and analogues of human FGF21 by specific and proportional expression of Firefly Luciferase.

Frequently Asked Questions


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