cTerminal FGF21 ELISA Kit
$680.00
The C-Terminal FGF-21 ELISA Assay Kit is intended for use in the quantitative determination of C-terminal FGF-21 levels in human serum and EDTA plasma. It measures both the intact FGF-21 and the C-terminal FGF-21 fragments that must not be C-terminally truncated while the N-terminal end of the FGF-21 may be truncated. The Eagle Biosciences C-Terminal FGF-21 ELISA Assay Kit is intended for research use only.
cTerminal FGF21 ELISA Kit
cTerminal FGF21 ELISA Kit is developed and manufactured in the USA
Size: 1×96 wells
Sensitivity: 3.7 pg/mL
Dynamic Range: 54.0 – 2100 pg/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 100 µL
Alternative Names: C-Terminal FGF-21, C-Terminal Fibroblast Growth Factor 21, cFGF21, cFGF 21
For Research Use Only
Controls Included
Assay Principle
The cTerminal FGF21 ELISA Kit is designed, developed and produced for the quantitative measurement of human C-terminal FGF-21 in serum and EDTA-plasma sample. The assay utilizes the two-site “sandwich” technique with two selected antibodies that bind to different epitopes of human FGF-21. One of the antibodies specifically binds to the C-terminal human FGF-21 (175-181) and the other is to the multi-epitopes of mid-regional and N-terminal human FGF-21.
Assay calibrators, controls and patient samples are added directly to wells of a microplate that is coated with an anti-human FGF-21 (175-181) specific antibody. After the first incubation period, a horseradish peroxidase-conjugated anti-human FGF-21 polyclonal antibody is added to each well. After the second incubation period, the antibody on the wall of microtiter well captures human C-terminal FGF-21 in the sample and further forms “sandwich” with the tracer antibody. Unbound proteins in each microtiter well are washed away. An immunocomplex of “anti-FGF-21 antibody — human C-terminal FGF-21 — HRP-conjugated tracer antibody” is formed. The unbound tracer antibody is removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to human C-terminal FGF-21 on the wall of the microtiter well is directly proportional to the amount of C-terminal FGF-21 in the sample. A calibrator curve is generated by plotting the absorbance versus the respective human intact FGF-21 concentration for each calibrator on point-to-point or 4 parameter curve fit. The concentration of human C-terminal FGF-21 in test samples is determined directly from this calibrator curve.
Related Products
Intact FGF-21
Total FGF-21
N-Terminal FGF-21
Documents
Product Documents
Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.
Publications
Citations
Parmar, Biraj, et al. “Eccentric Exercise Increases Circulating Fibroblast Activation Protein α but Not Bioactive Fibroblast Growth Factor 21 in Healthy Humans.” Experimental Physiology, vol. 103, no. 6, 1 June 2018, pp. 876–883., doi:10.1113/ep086669.
Soeberg S, et al. (2018). FGF21, a Liver Horomone That Inhibits Alcohol Intake in Mice, Increases in Human Circulation After Acute Alcohol Injestion and Sustained Binge Drinking at Oktober Fest. Molecular Metabolism.
Keipert, S;Kutschke, M;Ost, M;Schwarzmayr, T;van Schothorst, EM;Lamp, D;Brachthäuser, L;Hamp, I;Mazibuko, SE;Hartwig, S;Lehr, S;Graf, E;Plettenburg, O;Neff, F;Tschöp, MH;Jastroch, M;, (2017). Long-Term Cold Adaptation Does Not Require FGF21 or UCP1. Cell Metab., 26(1), 437-446.e5-
Shenoy, VK;Beaver, KM;Fisher, FM;Singhal, G;Dushay, JR;Maratos-Flier, E;Flier, SN;, (2016). Elevated Serum Fibroblast Growth Factor 21 in Humans with Acute Pancreatitis. PLoS ONE, 11(11), e0164351-
Singhal, G;Douris, N;Fish, AJ;Zhang, X;Adams, AC;Flier, JS;Pissios, P;Maratos-Flier, E;Fibroblast growth factor 21 has no direct role in regulating fertility in female mice; Molecular Metabolism.2016: 5(8):690-698.
Coppage, AL;Heard, KR;DiMare, MT;Liu, Y;Wu, W;Lai, JH;Bachovchin, WW; Human FGF-21 Is a Substrate of Fibroblast Activation Protein, PLoS ONE; 11:3:e0151269, doi: 10.1371/journal.pone.0151269
Keipert, S. et al. “Genetic disruption of uncoupling protein 1 in mice renders brown adipose tissue a significant source of FGF21 secretion.”Molecular Metabolism. 2015: 4(7):537-542.