Human VEGFR2/KDR ELISA Kit

$570.00

The Human VEGFR2/KDR ELISA Kit is intended for the quantitation of Human KDR concentrations in cell culture supernates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human VEGFR2/KDR ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: KDR31-K01 Categories: , ,

Human VEGFR2/KDR ELISA Kit

The Human VEGFR2/KDR ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <4pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Names: Vascular endothelial growth factor receptor 2, Kinase insert domain receptor


Assay Principle

The Human KDR Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KDR with a 96-well strip plate that is pre-coated with antibody specific for KDR. The detection antibody is a biotinylated antibody specific for KDR. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KDR with immunogen: Expression system for standard: NSO; Immunogen sequence: A20-E764. The kit is analytically validated with ready to use reagents. To measure Human KDR, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human KDR in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human KDR in the sample.


Related Products

Human sVEGFR1 ELISA Kit
Human FLT4/VEGFR3 ELISA Kit
Mouse VEGFR2/KDR ELISA Kit

Additional Information

Assay Background


Vascular endothelial growth factor receptor 2(VEGFR-2) also known as Kinase insert domain receptor(KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11–>q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.

Typical Standard Curve


Human VEGFR2/KDR ELISA Kit

Documents

Product Manual


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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