The Human sVEGFR1 ELISA Kit is intended for the quantitation of Human FLT1 concentrations in cell culture supernates, serum and plasma (EDTA). The Eagle Biosciences Human sVEGFR1/sFLT1 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: FLT31-K01 Categories: , ,


The Human sVEGFR1 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <30pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum and plasma(EDTA)
Sample Size: 100 µl
Alternative Names: sFLT1, sFMS-related tyrosine kinase 1

Assay Principle

The Human FLT1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FLT1 with a 96-well strip plate that is pre-coated with antibody specific for FLT1. The detection antibody is a biotinylated antibody specific for FLT1. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human FLT1 with immunogen: Expression system for standard: sf21; Immunogen sequence: S27-H687. The kit is analytically validated with ready to use reagents. To measure Human FLT1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FLT1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human FLT1 in the sample.

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Additional Information

Assay Background

sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.

Typical Standard Curve


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