Human Kallikrein-3 ELISA Kit

$550.00

The Human Kallikrein-3 ELISA Kit is intended for the quantitation of Human KLK3 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human Kallikrein 3 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: HK331-K01 Categories: , ,

Human Kallikrein-3 ELISA Kit

The Human Kallikrein-3 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 312pg/ml-20,000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Names: KLK3, Prostate-specific antigen, PSA, seminin, semenogelase, gamma-seminoprotein and P-30 antigen


Assay Principle

The Human KLK3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK3 with a 96-well strip plate that is pre-coated with antibody specific for KLK3. The detection antibody is a biotinylated antibody specific for KLK3. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KLK3 with immunogen: Expression system for standard: NSO; Immunogen sequence: A18-P261. The kit is analytically validated with ready to use reagents. To measure Human KLK3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human KLK3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human KLK3 in the sample.


Related Products

Human KLK5 ELISA Kit
Human KLK1 ELISA Kit
Human KLK13 ELISA Kit

Additional Information

Assay Background


Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3

Typical Standard Curve


Human Kallikrein-3 ELISA Kit

Documents

Product Documents