The Eagle Bioscience’s Human TGF-β3 is for research use only and not to be used for diagnostic procedures.
Produced in E.coli using a patented expression system.
TGF-β3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-β super family that share a characteristic cysteine-knot structure. TGF-β1, -β2 and -β3 are highly pleiotropic cytokines that regulate immune function, proliferation and epithelial mesenchymal transition. Each TGF-β isoform has some non-redundant functions. So mice with a targeted deletion of TGF-β3 show defects in palatogenesis and pulmonary development. Human TGF-β3 cDNA encodes a 412 amino acid precursor that contains a 20 amino acid signal peptide and a 392 amino acid precursor. A furin-like convertase processes the precursor to generate an N terminal 220 amino acid latency associated peptide and a C terminal 112 amino acid mature TGF-β3. Mature human TGF-β3 shows 100%, 99% and 98% identity with mouse/dog/horse, rat and pig TGF-β3, respectively.
- Purity: >95 % (as determined by HPLC analysis)
- Quantity: 20 µg, 100 µg
- Molecular Weight: 25,427 daltons- confirmed by Mass Spectrometry
- N-terminal sequence analysis: Analysis of 5 residues
- Biological Activity: Inhibition of proliferation in mink lung epithelial cells (Mv1Lu) (IC50 < 0.75 ng/ml)
- Endotoxin (LAL): <0.1 EU/ug
- Storage/Stability: At least 2 years at 2-4°C (lyophilized)
- Appearance: White powder freeze-dried with mannitol.