Mouse TGF-beta 2 ELISA Kit

$515.00

The Mouse TGF-beta 2 ELISA Kit is intended for the quantitation of Mouse TGFB2 concentrations in cell culture supernates, serum and plasma (heparin, EDTA, citrate). The Eagle Biosciences Mouse TGF-beta 2 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: TGF11-K01 Categories: , ,

Mouse TGF-beta 2 ELISA Kit

The Mouse TGF-beta 2 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA, citrate)
Sample Size: 100 µl
Alternative Name: Transforming growth factor-beta 2, TGFB2


Assay Principle

The Mouse TGFB2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse TGFB2 with a 96-well strip plate that is pre-coated with antibody specific for TGFB2. The detection antibody is a biotinylated antibody specific for TGFB2. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse TGFB2 with immunogen: Expression system for standard: NSO; Immunogen sequence: A303-S414. The kit is analytically validated with ready to use reagents. To measure Mouse TGFB2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse TGFB2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse TGFB2 in the sample.


Related Products

Mouse TGF-beta 3 ELISA Kit
Mouse TGF-beta 3 ELISA Kit
Mouse LIF ELISA Kit

Additional Information

Assay Background


Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Typical Standard Curve


Documents

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