Human SP-D ELISA Kit


The Human SP-D ELISA Kit is intended for the quantitation of Human SFTPD concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human SP-D ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

Human SP-D ELISA Kit

The Human SP-D ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <20pg/ml
Standard Range: 625pg/ml-40000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Names: Surfactant, pulmonary-associated protein D, SFTPD

Assay Principle

The Human SFTPD Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human SFTPD with a 96-well strip plate that is pre-coated with antibody specific for SFTPD. The detection antibody is a biotinylated antibody specific for SFTPD. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human SFTPD with immunogen: Expression system for standard: NSO; Immunogen sequence: A21-F375. The kit is analytically validated with ready to use reagents. To measure Human SFTPD, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human SFTPD in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human SFTPD in the sample.

Related Products

Human PSP94 ELISA Kit
Mouse SP-D ELISA Kit

Additional Information

Assay Background

Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Typical Standard Curve


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