Human PD-L2 ELISA Kit

$515.00

The Eagle Biosciences Human PD-L2/B7-DC ELISA Kit is intended for the quantitation of Human PDCD1LG2 concentrations in cell culture supernates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human PD-L2/B7-DC ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: PD231-K01 Categories: , ,

Human PD-L2 ELISA Kit

The Human PD-L2 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <12pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Name: B7-DC, Programmed cell death 1 ligand 2, PDCD1LG2


Assay Principle

The Human PDCD1LG2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PDCD1LG2 with a 96-well strip plate that is pre-coated with antibody specific for PDCD1LG2. The detection antibody is a biotinylated antibody specific for PDCD1LG2. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human PDCD1LG2 with immunogen: Expression system for standard: NSO, Immunogen sequence: L20-T220. The kit is analytically validated with ready to use reagents. To measure Human PDCD1LG2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human PDCD1LG2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human PDCD1LG2 in the sample.


Related Products

Human PD-L1/B7-H1 ELISA Kit
Mouse PD-L1/B7-H1 ELISA Kit
Human MCAM/CD146 ELISA Kit

Additional Information

Assay Background


Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is a protein that in humans is encoded by the PDCD1LG2 gene. It is mapped to chromosome 9p24.2. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. Northern blot analysis detect wide expression of PDL2, with highest levels in placenta, heart, pancreas, lung, and liver, and lower levels in spleen, lymph nodes, and thymus. PDL2 negatively regulates T cells and plays an essential role in immune tolerance.

Typical Standard Curve


Human PD-L2 ELISA Kit

Documents

Product Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations