Human Ki67 ELISA Kit

$500.00

The Eagle Biosciences Human Ki67 ELISA Kit is intended for the quantitation of Human MKI67 concentrations in cell culture supernates, serum and plasma (heparin, EDTA, citrate). The Eagle Biosciences Human Ki67 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

Human Ki67 ELISA Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: <1pg/ml
Standard Range: 7.8pg/ml-500pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Sample Size: 100 µl

Additional Information

Assay Background


Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. This gene is mapped to 10q26.2. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Assay Principle


The Human MKI67 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MKI67 with a 96-well strip plate that is pre-coated with antibody specific for MKI67. The detection antibody is a biotinylated antibody specific for MKI67. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human MKI67 with immunogen: . The kit is analytically validated with ready to use reagents. To measure Human MKI67, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MKI67 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human MKI67 in the sample.

Typical Standard Curve


Manual

Product Manual