Human PSP94 ELISA Kit

$515.00

The Eagle Biosciences Human PSP94 ELISA Kit is intended for the quantitation of Human MSMB concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human PSP94 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: PSP31-K01 Categories: , ,

Human PSP94 ELISA Kit

The Human PSP94 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Name: Beta-microseminoprotein, MSMB, Prostate secretory protein 94, microseminoprotein, beta-inhibitin, prostatic inhibin peptide, inhibitin like material


Assay Principle

The Human MSMB Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MSMB with a 96-well strip plate that is pre-coated with antibody specific for MSMB. The detection antibody is a biotinylated antibody specific for MSMB. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MSMB with immunogen: Expression system for standard: E.coli; Immunogen sequence: S21-I114. The kit is analytically validated with ready to use reagents. To measure Human MSMB, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MSMB in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human MSMB in the sample.


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Additional Information

Assay Background


Beta-microseminoprotein, also known as MSMB or PSP94, is a protein that in humans is encoded by the MSMB gene. It is a member of the immunoglobulin binding factor family. This gene is mapped to 10q11.23. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. The expression of the encoded protein is found to be decreased in prostate cancer. But transcripts of identical size were found in prostate, respiratory(tracheal, bronchial, and lung) tissues, and the mucosa of the gastric antrum. Thus, the protein may have functions other than those related to male reproduction. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues.

Typical Standard Curve


Human PSP94 ELISA Kit

Documents

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