Human Recombinant MANF Protein

$460.00$1,685.00

The Eagle Biosciences Human Recombinant MANF Protein is for research use only. MANF trophic factor for midbrain dopamine neurons in vivo prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent with Parkinson’s disease.

SKU: P-101-100 Categories: ,

Human Recombinant MANF Protein

The Human Recombinant MANF Protein is For Research Use Only

Synonyms: Mesencephalic astrocyte-derived neurotrophic factor – MANF; ARMET; ARP
Uniprot ID: P55145
Source: Human
Host: CHO-based cell line (expressed by QMCF Technology)
Buffer: PBS pH 7.4
Endotoxine: Less than 1EU/mg of protein as determined by LAL method


Molecular Weight:
Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids

Purification:
Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.

Concentration:
1 mg/ml   Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard.

Bioproperties:
Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat 6-OHDA model of Parkinson’s disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke.

Quality Control:
SDS-PAGE and Western-Blot analysis (Figure 1 and 2); Mass-spectroscopy: Purified human MANF is characterized as homogenous material by mass-spectroscopy

Background:
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Shipping:
Shipped in dry-ice

Storage:  
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities.


Related Products

MANF ELISA Assay Kit
Human MANF Rabbit Polyclonal Antibody
Human MANF Mouse Monoclonal Antibody
Product Developed and Manufactured by Icosagen

Additional Information

Molecular Weight:
Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids

Purification:
Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.

Concentration:
1 mg/ml   Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard.

Bioproperties:
Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat 6-OHDA model of Parkinson’s disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke.

Quality Control:
SDS-PAGE and Western-Blot analysis (Figure 1 and 2); Mass-spectroscopy: Purified human MANF is characterized as homogenous material by mass-spectroscopy

Background:
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Shipping:
Shipped in dry-ice

Storage:  
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities.

Related Products:  
Polyclonal and monoclonal antibodies against human MANF.  MANF ELISA Assay Kit.

Figure 1. SDS-PAGE analysis of human MANF visualized by PageBlue protein staining solution (Fermentas). Line 1. Protein size marker (PageRuler Prestained protein ladder, Fermentas); Line 2. 10 µg of MANF.

Figure 2. Western Blot testing of human MANF using anti-MANF polyclonal antibody (Eagle Biosciences Cat. No 310-100). Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant MANF expressed into the supernatant of CHO cell culture medium.

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