Human Recombinant MANF Protein
The Human Recombinant MANF Protein is For Research Use Only
Synonyms: Mesencephalic astrocyte-derived neurotrophic factor – MANF; ARMET; ARP
Uniprot ID: P55145
Host: CHO-based cell line (expressed by QMCF Technology)
Buffer: PBS pH 7.4
Endotoxine: Less than 1EU/mg of protein as determined by LAL method
Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids
Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard.
Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat 6-OHDA model of Parkinson’s disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke.
SDS-PAGE and Western-Blot analysis (Figure 1 and 2); Mass-spectroscopy: Purified human MANF is characterized as homogenous material by mass-spectroscopy
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Shipped in dry-ice
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities.