Human Recombinant CDNF Protein


The Eagle Biosciences Human Recombinant CDNF Protein is for research use only.

SKU: P-100-100 Categories: ,

Human Recombinant CDNF Protein

The Human Recombinant CDNF Protein is For Research Use Only

Synonyms: Cerebral dopamine neurotrophic factor – CDNF; ARMETL1
Uniprot ID: Q49AH0
Source: Human
Host: CHO-based cell line (expressed by QMCF Technology)
Buffer: PBS pH 7.4
Endotoxine: Less than 1EU/mg of protein as determined by LAL method

Molecular Weight:
Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids

Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media. Protein is sterile-filtrated through 0.22 µg filter.

1 mg/ml   Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard.

Protein is biologically active. Suitable for cell culture and in vivo testing. Biological activity of human CDNF was tested in rat 6-OHDA model of Parkinson’s disease and shown to be both neuroprotective and neurorestorative (for methods see: Lindholm et al. 2007; Voutilainen et al. 2011).

Quality Control: 
SDS-PAGE and Western-Blot analysis (Figure 1 and 2, respectively).  Mass-spectroscopy: Purified human CDNF is characterized as homogenous material by mass-spectroscopy.


CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Shipped in dry-ice

Store at -70°C upon receipt. Recommended to aliquot into smaller quantities.

Related Products

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Product Developed and Manufactured by Icosagen

Additional Information



Human Recombinant CDNF Protein









Figure 1. SDS-PAGE analysis of human CDNF visualized by PageBlue protein staining solution (Fermentas). Line 1. 4 µg of CDNF; Line 2. Protein size marker (PageRuler Prestained protein ladder, Fermentas).


Human Recombinant CDNF Protein










Figure 2. Western Blot testing of human CDNF using anti-CDNF polyclonal antibody (Eagle Biosciences Cat. No 300-100). Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant CDNF expressed into the supernatant of CHO cell culture medium.

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