Human Recombinant CDNF Protein

$460.00$1,685.00

The Eagle Biosciences Human Recombinant CDNF Protein is for research use only.

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Human Recombinant CDNF Protein

The Human Recombinant CDNF Protein is For Research Use Only

Synonyms: Cerebral dopamine neurotrophic factor – CDNF; ARMETL1
Uniprot ID: Q49AH0
Source: Human
Host: CHO-based cell line (expressed by QMCF Technology)
Buffer: PBS pH 7.4
Endotoxine: Less than 1EU/mg of protein as determined by LAL method


Molecular Weight:
Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids

Purification: 
Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media. Protein is sterile-filtrated through 0.22 µg filter.

Concentration:
1 mg/ml   Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard.

Bioproperties:
Protein is biologically active. Suitable for cell culture and in vivo testing. Biological activity of human CDNF was tested in rat 6-OHDA model of Parkinson’s disease and shown to be both neuroprotective and neurorestorative (for methods see: Lindholm et al. 2007; Voutilainen et al. 2011).

Quality Control: 
SDS-PAGE and Western-Blot analysis (Figure 1 and 2, respectively).  Mass-spectroscopy: Purified human CDNF is characterized as homogenous material by mass-spectroscopy.

Background:

CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Shipping:
Shipped in dry-ice

Storage:
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities.


Related Products

CDNF ELISA Assay Kit
Human CDNF Mouse Monoclonal Antibody
Human CDNF Rabbit IgG Polyclonal Antibody

Product Developed and Manufactured by Icosagen

Additional Information

 

 

 

 

 

 

 

 

 

 

Figure 1. SDS-PAGE analysis of human CDNF visualized by PageBlue protein staining solution (Fermentas). Line 1. 4 µg of CDNF; Line 2. Protein size marker (PageRuler Prestained protein ladder, Fermentas).

 

 

 

 

 

 

 

 

 

 

Figure 2. Western Blot testing of human CDNF using anti-CDNF polyclonal antibody (Eagle Biosciences Cat. No 300-100). Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant CDNF expressed into the supernatant of CHO cell culture medium.

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