Human Recombinant Artemin Protein
The Human Recombinant Artemin Protein is For Research Use Only
Synonyms: Artemin, Enovin, Neublastin, ARTN
Uniprot ID: Q5T4W7
Host: CHO-based cell line (expressed by QMCF Technology)
Buffer: PBS pH 7.4
Endotoxine: Less than 1EU/mg of protein as determined by LAL method
Approximately 18.8 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids. Protein is homodimer.
Purified from serum-free CHO growth media by ion-exchange chromatography and gel-filtration. Protein is sterile-filtered through 0.22 µm filter.
0.5 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard.
Product is suitable for cell culture and in vivo testing. Protein is biologically active. Biological activity of purified artemin was tested in in vitro receptor-dependent cellular morphology change induction assay using U2OS cells expressing Ret and GFRα-3 receptor complex.
SDS-PAGE and Western-Blot analysis
Artemin is a ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer’s patch-like structures, a major component of the gut-associated lymphoid tissue (www.uniprot.org; Baloh et al. 1998, Neuron; Rosenblad et al. 2000, Eur. J. Biochem).
Shipped in dry-ice
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities.
Product Developed and Manufactured by Icosagen