Human MANF Mouse Monoclonal Antibody


Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant

SKU: 311-100 Categories: ,

Human MANF Mouse Monoclonal Antibody

The Human MANF Mouse Monoclonal Antibody is For Research Use Only

Immunogen: Human MANF
Uniprot ID: P55145
Alternative Names: ARMET, ARP
Clone: 1G12
Clonality: Mouse monoclonal
Class: IgG1
Reactivity: Human MANF
Application: ELISA, WB
Purification: IgG fraction purified with Protein G affinity chromatorgaphy
Buffer: PBS pH 7.4, with 0.1% sodium azide
Concentration: 1 mg/ml
Unit Size: 100 µg
Related Products: Monoclonal and polyclonal antibodies to human MANF.
Shipping: This product is shipped in non-frozen liquid form in ambient conditions.
Storage: Store at –20 to -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
Alternative Names: Mesencephalic astrocyte-derived neurotrophic factor

Protocol: ELISA 1:32 000; Western immunoblotting 1:2000 – 1:4000
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Background: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta

Related Products

Human MANF Rabbit Polyclonal Antibody
Human Recombinant MANF Protein

Product Developed and Manufactured by Icosagen

Additional Information

Figure 1:
Human MANF Mouse Monoclonal Antibody

Western Blot testing of anti-MANF monoclonal antibody. Line 1. Page Ruler prestained protein ladder, Fermentas; Line 2. Purified recombinant MANF (500 ng per line) expressed using CHOEBNALT85  cell line.

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