Human MANF Rabbit Polyclonal Antibody

$535.00

Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant.

SKU: 310-100 Categories: ,

Human MANF Rabbit Polyclonal Antibody

The Human MANF Rabbit Polyclonal Antibody is For Research Use Only

Immunogen: Human MANF
Uniprot ID: P55145
Alternative Names: ARMET, ARP
Clonality: Rabbit polyclonal
Class: IgG
Reactivity: Human MANF
Application: ELISA, WB
Purification: MANF-affinity purified
Buffer: Concentrated ammonium sulphate in PBS pH 7.4.
Concentration: 0.45 mg/ml
Unit Size: 100 µg
Related Products: Monoclonal and polyclonal antibodies to human MANF.
Shipping: This product is shipped in non-frozen liquid form in ambient conditions.
Storage: Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Alternative Names: Mesencephalic astrocyte-derived neurotrophic factor


Protocol: ELISA 1:200 000; Western immunoblotting 1:2000 – 1:6000, IHC, IF
No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.   Polyclonal antibody working titer has to be established practically for each particular antigen and assay format.

Background: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta


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Product Developed and Manufactured by Icosagen

Additional Information

Figure 1:

Western Blot testing of anti-MANF polyclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (1 µg per line) expressed using CHOEBNALT85  cell line.

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