Human Recombinant MANF Protein

Additional Information

Molecular Weight:
Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids

Purification:
Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.

Concentration:
1 mg/ml   Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard.

Bioproperties:
Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat 6-OHDA model of Parkinson’s disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke.

Quality Control:
SDS-PAGE and Western-Blot analysis (Figure 1 and 2); Mass-spectroscopy: Purified human MANF is characterized as homogenous material by mass-spectroscopy

Background:
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Shipping:
Shipped in dry-ice

Storage:
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities.

Related Products:
Polyclonal and monoclonal antibodies against human MANF.  MANF ELISA Assay Kit.

Figure 1. SDS-PAGE analysis of human MANF visualized by PageBlue protein staining solution (Fermentas). Line 1. Protein size marker (PageRuler Prestained protein ladder, Fermentas); Line 2. 10 µg of MANF.

Figure 2. Western Blot testing of human MANF using anti-MANF polyclonal antibody (Eagle Biosciences Cat. No 310-100). Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant MANF expressed into the supernatant of CHO cell culture medium.