Human Pro-IGF-I EB (104 – 123)
$485.00
The Eagle Bioscience’sHuman Pro-IGF-I EB (104 – 123) is for research use only and not to be used for diagnostic procedures.
Human Pro-IGF-I EB (104 – 123)
The Eagle Bioscience’s Human Pro-IGF-I EB (104 – 123) is for research use only and not to be used for diagnostic procedures.
Product Specifications
Source:
Rabbit antiserum
Description:
Human ProIGF-I EB (104-123) antiserum was prepared by immunizing rabbits with a 21 amino acid peptide derived from human ProIGF-I EB linked to diphtheria toxoid. The peptide is located within the E domain of the 195 amino acid ProIGF-I EB, derived from alternative splicing of the human igf-1 gene. The antiserum recognizes ProIGF-I EB but not ProIGF-I EA, ProIGF-I EC, mature IGF-I or IGF-II. Peptide sequence analysis suggest it may recognize ProIGF-I EB related proteins in a range of nonhuman primates but not many other species due to alternative splicing of the igf-1 gene. There is significant homology to similar regions of ProIGF-I variants in bovine, porcine and canine species to suggest it may be effective.
- Immunogen: Derived from UniProtKB/Swiss-Prot sequence ID P05019 isoform 1 also known as IGF-IB which is recognized as the canonical sequence. The peptide sequence is amino-acids 151-170, derived from ProIGF-I EB, conjugated to diphtheria toxoid.
- Quantity: 100 µg
- Specificity: Recognizes Human ProIGF-I EB (isoform 1, P05019). No cross-reactivity against ProIGF-I EA (isoform 2, P05019-2); ProIGF-I EC (Q13429) mature IGF-I or IGF-I.
- Appearance:Lyophilized powder
- Storage/Stability: At least 2 years at 2 – 4°C (lyophilized). After reconstitution store at -20°C or -80°C. Avoid freeze-thaw cycles
- Reconstitution:Dissolve the vial contents in 100 µl sterile water and then dilute in a buffer suitable for the application.
- Application and Titre:Each application and titre should be determined in house. As a guideline the antiserum can be used at a dilution of 1:1000 for Western immunoblotting.
Related Products
Package Inserts
Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.
